Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Pandemic driven innovation: A pilot evaluation of an alternative respiratory pathogen collection deviceArticle Published on 2022-11-012022-11-16 Journal: The American journal of emergency medicine [Category] SARS, 유전자 메커니즘, [키워드] absence All participant amplification communicable disease COVID-19 demonstrated determine diagnostic enrolled evaluated faster favor feedback fused gold standard identify inhibitor Innovation Interpretation invasive mechanism nasopharyngeal Nasopharyngeal swab nucleic acids participant Patient PCR PCR cycle PCR testing pilot study polymerase chain positive producing real-time reverse transcriptase receiving respiratory respiratory pathogen respiratory virus Result resulting RNase P RNAseP RT-qPCR Self-collection respiratory pathogen device. specimen study participant TaqPath tested threshold [DOI] 10.1016/j.ajem.2022.08.047 PMC 바로가기
Infection and Transmission of SARS-CoV-2 B.1.617.2 Lineage (Delta Variant) among Fully Vaccinated IndividualsArticle Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Alpha variant analysis Asia association B.1.1.7 lineage B.1.617.2 Breakthrough infection breakthrough infection. circulating Cluster Complete conducted coronavirus disease COVID-19 Delta delta variant demonstrated determine diagnostic dominant epidemiologically evaluate Evidence Factor genomic genotyping highest increase in independent individual individuals Infection Infectious virus information Isolation Lineage New York PCR peaked performed phylogenetic clusters positive real-time reverse transcriptase Replication SARS-CoV-2 SARS-CoV-2 lineage secretion sequence spread to subset Surveillance the SARS-CoV-2 Transmission transmission clusters transmission of SARS-CoV-2 vaccinated individual vaccinated individuals vaccination rate Viral load viral RNA load viral titer virological assessment virus Virus titration VoC [DOI] 10.1128/spectrum.00563-22 PMC 바로가기
When to test for COVID-19 using real-time reverse transcriptase polymerase chain reaction: a systematic reviewReview article Published on 2022-10-012022-10-05 Journal: International Journal of Infectious Diseases [Category] 치료기술, [키워드] COVID-19 addition affected analyzed article Case series cohorts conducted coronavirus cough country COVID-19 COVID-19 testing Cross-sectional studies database described Dyspnea evaluate Fever first positive health units Infection initial symptom median age objective participant Patient patients polymerase chain positive positive test result real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction reported Result retrospective cohorts RT-PCR RT-PCR test RT-PCR testing SARS-CoV-2 Symptom symptom onset systematic review the MEDLINE [DOI] 10.1016/j.ijid.2022.06.037 [Article Type] Review article
Clinical characteristics and treatment outcomes of SARS-CoV-2 delta variant outbreak, Pingtung, Taiwan, June 2021Article Published on 2022-09-012022-11-15 Journal: Journal of the Formosan Medical Association = Taiw [Category] SARS, 변종, 치료제, [키워드] antiviral agent Antiviral agents Aspartate aminotransferase AST Characteristics clinical Clinical characteristics Coronavirus disease 2019 cough COVID-19 cycle threshold defined Delta delta variant demonstrated Diagnosis disease evaluated ferritin Fever headache hospital Infection lactate dehydrogenase LDH mechanical ventilation median Mortality occurred outbreak Patient Pingtung Pneumonia polymerase chain real-time reverse transcriptase repeated risk factor RT-PCR SARS-CoV-2 SARS-CoV-2 delta SARS-COV-2 infection serum Symptom symptomatic Taiwan Taiwan. Treatment Treatment outcome Two patient variant viral shedding [DOI] 10.1016/j.jfma.2022.01.008 PMC 바로가기
Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern진화하는 바이러스에 대한 진화하는 전략: 우려되는 모든 SARS-CoV-2 변이체를 검출하기 위한 실시간 PCR 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] Alpha Amino acid amino acid change amino acid changes approach B.1.1.7 Beta Canada characterized clinical sensitivity clinical specificity complementary COVID-19 Critical cross-reactivity Delta detect development discriminatory mutation E gene E484K FIVE Gamma Genome sequencing implementation K417N L452R less limit of detection Lineage molecular MONITOR N501Y Negative predictive value nucleotide omicron Omicron variants P681 Pathogens PCR PCR assays positive Positive predictive value positive predictive values positive sample predictive values reaction real-time PCR assay real-time reverse transcriptase real-time RT-PCR replacement respiratory respiratory pathogen responsible reverse transcriptase rRT-PCR SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genome sequencing SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Single nucleotide polymorphism single nucleotide polymorphisms specificity Spike protein subset Surveillance target the SARS-CoV-2 Variability variant variants variants of concern variants of concern. VoC VOCs while wild-type Δ69-70 [DOI] 10.1016/j.jviromet.2022.114553 PMC 바로가기 [Article Type] Article
The prognostic value of biomarker levels and chest imaging in patients with COVID-19 presenting to the emergency department응급실에 제출하는 COVID-19 환자의 바이오마커 수치 및 흉부 영상의 예후 가치Article Published on 2022-09-012022-09-11 Journal: The American journal of emergency medicine [Category] COVID19(2023년), MERS, SARS, 바이오마커, [키워드] 95% CI age AUC Biomarker Biomarkers C-reactive protein Care Chain Reaction Chest CT chest imaging COVID-19 COVID-19 patient COVID-19 patients CRP D-dimer Department dimer Emergency emergency department ferritin Hospital mortality ICU admission in-hospital mortality Laboratory parameters Lactate lactate dehydrogenase LDH Logistic regression Lung imaging lymphocyte mechanical ventilation Mortality Natriuretic neutrophil Neutrophil to lymphocyte ratio. neutrophil/lymphocyte ratio NLR not performed obesity Patient patients with COVID-19 peptide performed polymerase chain polymerase chain reaction positive presenting Primary outcome procalcitonin prognostic Prognostic value Quantitative real-time reverse transcriptase retrospective reverse transcriptase Reverse transcriptase polymerase chain reaction severity significantly tested troponin vital sign [DOI] 10.1016/j.ajem.2022.06.043 PMC 바로가기 [Article Type] Article
Assessment of Clinical and Virological Characteristics of SARS-CoV-2 Infection Among Children Aged 0 to 4 Years and Their Household MembersArticle Published on 2022-08-012022-11-15 Journal: JAMA Network Open [Category] SARS, 변종, 치료기술, [키워드] 95% CI Adults age Alpha variant analyses antibodies Asian assessment Asymptomatic black Characteristics children clinical Cohort collected Community correlated correlation Delta delta variant enrolled Enrollment feature Features female Frequency greater help highest Hispanic household incidence individual Infection Lineage male median member nasal swab not differ nucleocapsid protein number of symptom Older outbreak outcome participant polymerase chain Prevent prospective cohort study Quantitative questionnaire real-time reverse transcriptase RT-PCR RT-PCR testing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 lineage SARS-CoV-2 spike significantly submitted Surveillance Symptom symptomatic individual Symptoms variant variants of interest Viral load Virological white Year [DOI] 10.1001/jamanetworkopen.2022.27348 PMC 바로가기
Cycle threshold values and SARS-CoV-2: Relationship to demographic characteristics and disease severity주기 임계값 및 SARS-CoV-2: 인구 통계학적 특성 및 질병 중증도와의 관계Article Published on 2022-08-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Abbott age amplify Analysis association Care Characteristics complex contribute Ct value cycle cycle threshold demographic characteristics demonstrated detectable disease disease severity emergency department Epidemiology epidemiology. estimate Ethnicity Factor Kruskal-Wallis less multivariable logistic regression nasopharyngeal no significant difference no significant differences Odds ratio outcome outcomes Patient PCR PCR testing Previous studies previous study provide real-time reverse transcriptase relationship required Retrospective study reverse transcriptase risk rRT-PCR rRT-PCR testing SARS-CoV-2 SARS-COV-2 infection severe disease Sex symptom onset tested threshold threshold value univariable Viral load Viral RNA were used [DOI] 10.1002/jmv.27752 PMC 바로가기 [Article Type] Article
Gamma variant vertically transmitted from a mild symptomatic pregnant woman associated with fatal neonatal COVIDCase report Published on 2022-07-012022-10-05 Journal: The Brazilian journal of infectious diseases : an [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] age coronavirus COVID COVID 19 death Gamma homology Hospital admission Infection Inflammatory knowledge Mild mother Neonatal Newborn P.1 polymerase chain Pregnancy Pregnant woman Preterm infant progressed real-time reverse transcriptase SARS-CoV-2 SARS-CoV-2 variant Sequencing severe respiratory disease symptomatic symptomatic infection syndrome Transmission transmitted variant vertical transmission [DOI] 10.1016/j.bjid.2022.102385 [Article Type] Case report